67. One possible versionIn his diary, the man mentioned he wished to sink to the ocean with his wife but fortheir daughter he had to live. Without him, their baby daughter would become an orphan.After drowning himself in sorrow for a short time, he uplifted himself and learned how toraise his baby. Whenever faced with hardships, he would inspire himself with his wifewords-"Take care of our child. " Seeing the little girl grow into a sunny, pretty girl withdouble love from both parents, the man knew he made it to keep his promise.The story was finished and the class was silent. Nobody talked or laughed as usualInstead, it seemed that every student was lost in deep thought, tasting the moving life story.The teacher was sure that the students were reflecting on their replies and that they hadunderstood the moral of the story. Of the Good and Evil in this world, there are hiddensecrets behind them. So we should not really focus on the surface and judge anyone withoutunderstanding them first. Put yourself in others' shoes and then you can really understandtheir behavior

38.(1)①②③④(不全不给分)(2分)(2)提高(2分)(3) BamH I和HindⅢ(2分)若选 EcoR I会破坏质粒的复制原点(1分);若选SmaI和HindⅢ,则构建的重组质粒没有标记基因或复制原点(2分)(4)显微注射法(2分)桑椹胚或囊胚(2分)性别、年龄(2分)解析:(1)作为载体必须具有四个条件:①在宿主细胞中能保存下来并能大量复制;②有多个限制酶切点,而且每种酶的切点最好只有一个,可适于多种限制酶切割的DNA插入;③有一定的标记基因,便于选行筛选。④对受体细胞无害。一般来说,天然载体往往不能满足上述要求,因此需要根据不同的目的和需要,对载体进行人工改建,一个基因表达载体的组成,除了目的基因外,还必须有启动子、终止子以及标记基因等,因此经改造后的质粒同时具备的条件有①②③④,起始密码子位于mRNA上,不存在于质粒中,(2)SmaI的酶切位点是CCC·GGG,而双链中C-G互补配对形成3个氢键,A一T互补配对形威2个氫键,若插入的SmaI酶切位点越多,质粒的 CCCGGG序列就越多,热稳定性将会提高(3)用图2中的人乳铁蛋白目的基因和图1所示的质粒构建重组质粒时,选用的服制酶是BamH I和HindⅢ,既可以获得目的基因,也可以保留标记基因—-Ampr,且不破坏复制原点。若选EcoRⅠ会破坏质粒的复制原点;若选SmaI和HindⅢ,则构建的重组质粒没有标记基因或复制原点。(4)目的基因导入动物细胞最常用和最有效的方法是显微注射法③过程为胚胎移植,选用的早期胚胎应发育到桑褴胚或寰胚阶段。乳腺生物反应器是从乳汁中提取产品,所以必须利用怀孕的雌性个体,膀胱生物反应器是从尿液中提取产品,不受动物的性别、年龄限制

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